| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Thiobacillus denitrificans | TGCTCGATGCGCTGTTGT | AGCGTCGGCATCATCGAT | 60.36 | 59.27 | 166 | 141 |
100.00%
|
100.00%
|
| Primer 2 | Thiobacillus denitrificans | TGACGCCAACACGCTGAT | ACGGTTTCACCTGCGGAT | 59.97 | 59.25 | 212 | 141 |
100.00%
|
100.00%
|
| Primer 3 | Thiobacillus denitrificans | TGACGCCAACACGCTGAT | GACGGTTTCACCTGCGGAT | 59.97 | 60.38 | 213 | 141 |
100.00%
|
100.00%
|
| Primer 4 | Thiobacillus denitrificans | TGACGCCAACACGCTGAT | GTTCGACGGTTTCACCTGC | 59.97 | 59.43 | 217 | 141 |
100.00%
|
100.00%
|
| Primer 5 | Thiobacillus denitrificans | TGACGCCAACACGCTGAT | GCAGGTTCGACGGTTTCAC | 59.97 | 59.43 | 221 | 141 |
100.00%
|
100.00%
|
| Primer 6 | Thiobacillus denitrificans | TGACGCCAACACGCTGAT | ACGGTTTCACCTGCGGATG | 59.97 | 60.67 | 212 | 141 |
100.00%
|
100.00%
|
| Primer 7 | Thiobacillus denitrificans | CCGGAACTCGAATTGCAGC | ACGCAACGAAGCACAGGA | 59.57 | 59.89 | 223 | 140 |
100.00%
|
100.00%
|
| Primer 8 | Thiobacillus denitrificans | TCGCCAAGGTCCTGAAGGA | ACGCAACGAAGCACAGGA | 60.54 | 59.89 | 288 | 140 |
100.00%
|
100.00%
|
| Primer 9 | Thiobacillus denitrificans | CCGAGATCAATCGCCTGGA | ATGACGCACCTGCAGCAT | 59.26 | 60.05 | 209 | 140 |
100.00%
|
100.00%
|
| Primer 10 | Thiobacillus denitrificans | TGACTCAAGCCGTGCTGT | TGAAGAACTGGGTGCGCA | 59.18 | 59.49 | 153 | 138 |
100.00%
|
100.00%
|